ANO6 Knockout cell line （293T）

ANO6 Knockout cell line （293T） Order

Catalog#

YKO-H930

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

ANO6 Knockout cell line (293T)

Morphology

Epithelial-like, adherent

Passage ratio

1:3~1:6

Species

Human

Gene Name

ANO6

Gene ID

12800

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

90% DMEM+10%FBS

Target region

Knockout region: Exon 5 (based on transcript ANO6-201)
Validation: PCR+Sanger Sequencing

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:293T			STR Info (Cell bank) Cell Line:HEK293T
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	8	9		8	9
D13S317	12	12		12	12
D7S820	11	11		11	11
D16S539	9	13		9	13
VWA	16	19		16	19
TH01	7	9.3		7	9.3
AMEL	X	X		X	X
TPOX	11	11		11	11
CSF1PO	11	12		11	12

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the ANO6 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the 293T cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, ANO6 gene knockout ANO6 Knockout cell line (293T) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

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